Alverno Laboratories
Test Information & Preparation

24 Hour Urine Collection

PURPOSE OF TEST
Your doctor has ordered a test which requires the collection of all urine passed in a 24-hour period. The accurately timed urine collection is an important part of your examination. Medical decisions and treatment may depend on these results. This test is only valid IF the collection includes all urine passed in a 24-hour period. The test will be inaccurate and may have to be repeated if, for any reason, some of the urine passed during the 24-hour period is not put into the container for the collection. Your doctor may have ordered multiple urine tests. It is possible additional collections will be needed if the additional test(s) require a different preservative.

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COLLECTION OF SPECIMEN

  1. Obtain the 24-hour urine container from the laboratory. The lab will need a copy of the physician order to determine the proper preservative for your collection. The laboratory staff will add the appropriate preservative prior to the collection and will explain any precautions to you.
    • Note: DO NOT urinate directly into the container during the collection process. Use a clean container to collect your urine and then pour the specimen into the 24-hour urine container.
  2. At the beginning of the test, note the time. Empty your bladder by urinating directly into the toilet. Do not add this urine to the collection container. All urine passed from this point must be added to the 24-hour urine container for the duration of 24 hours.
    • For example, if you empty your bladder at 7am, all urine will be collected after that time up to and including 7am the following morning.

LABELING THE SPECIMEN

Print your full name and the date and time you started and stopped collecting the specimen on the urine container.

STORAGE OF SPECIMEN/DELIVERY TO LAB

Refrigerate the specimen during the collection and until it can be delivered to the laboratory.

If you have any questions about these instructions, please call the Client Service department at 219-989-3700 or 800-937-5521.

Clean Catch Midstream Urine Specimen

PURPOSE OF TEST
Your doctor has ordered a test which requires you to collect a midstream urine specimen. A carelessly collected urine specimen could give inconclusive results that require a repeat collection, thus delaying treatment, or could cause the wrong treatment to be given. In order to correctly determine whether or not any infecting organisms are present in the urine, the specimen must be free of any contaminating organisms which might be present around the genital region. It is therefore important to collect a clean-catch urine.

Read Instructions
COLLECTION OF SPECIMEN

  1. Obtain the collection cup from the laboratory. Ensure your name and DOB are correct on the container.
  2. Wash your hands thoroughly with soap and warm water.
  3. If provided, open the antimicrobial towelette and wipe the external genital area.
  4. Open the collection cup without touching the inside of the cup or lid.
  5. Collect the sample by beginning to void into the toilet. While voiding, move the container into the stream of urine to collect the sample. Fill the cup approximately one half full. Finish urinating into the toilet.
  6. Close the container tightly and bring to the laboratory immediately. If transport is delayed, please refrigerate sample, and deliver to laboratory within 2 hours.

IF YOU HAVE ANY QUESTIONS ABOUT THESE INSTRUCTIONS, PLEASE ASK THE TECHNICIAN OR NURSE BEFORE COLLECTING THE SAMPLE.

Pediatric Urine Collection

PURPOSE OF TEST
Your doctor has ordered a test on your infant/child which requires you to collect a midstream urine specimen. A carelessly collected urine specimen could give inconclusive results that require a repeat specimen thus delaying treatment, or could cause the wrong treatment to be given. In order to correctly determine whether or not any infecting organisms are present in the urine, the specimen must be free of any contaminating organisms which might be present in or about the genital region. It is therefore important to collect a clean-catch urine.

Read Instructions
COLLECTION OF SPECIMEN

  1. Obtain a pediatric urine bag and a clean urine container from the laboratory.
  2. Wash your hands thoroughly with soap and warm water.
  3. Use soap and water to clean the genital and anal area of your child thoroughly. Pat dry.
  4. Peel the waxed paper off the urine bag and gently press the opening over the genital area. Make sure the bag is firmly attached to the skin to avoid leakage.
  5. Leave the bag in place until it is 1/3 full; however, do not leave the bag in place for more than 1 ½ hours.
  6. Gently remove the bag from the infant/child’s skin and place the urine filled bag into the urine container.
  7. Close the container tightly and bring the specimen to the laboratory immediately.

LABELING THE SPECIMEN

Print your child’s full name and the date and time the specimen was collected on the specimen container.

STORAGE OF SPECIMEN/DELIVERY TO LAB

Close the container tightly and bring to the laboratory immediately. If transport is delayed, please refrigerate sample and deliver to the laboratory within 2 hours.

If you have any questions about these instructions, please call the Client Service department at 219-989-3700 or 800-937-5521.

Pinworm Exam

PURPOSE OF TEST
Your doctor has ordered this test to determine if Pinworm eggs are present. During the night, the female worm will lay eggs around the opening of the anus.

Read Instructions
COLLECTION OF SPECIMEN

  1. Obtain the special pinworm kit from the laboratory. The kit contains a plastic paddle with a sticky surface on one side.
  2. Collect the specimen in the morning before arising, bathing, or having a bowel movement.
  3. To collect the specimen:
    • Remove the cap from the plastic tube.
    • Remove the paddle from the tube and note the side marked “Sticky Side”. For accurate results, DO NOT touch the sticky side of the paddle with your fingers.
    • Press the sticky side of the paddle against the area around the anus and then remove carefully.
    • Replace the paddle inside the plastic tube and put the cap back on.

LABELING THE SPECIMEN

Print the patient’s full name and the date and time the specimen was collected on the container.

STORAGE OF SPECIMEN/DELIVERY TO LAB

Return the tube containing the paddle to the laboratory. The tube does not need to be stored in the refrigerator.

If you have any questions about these instructions, please call the Client Service department at 219-989-3700 or 800-937-5521.

Fasting Specimens

PURPOSE OF TEST
Your doctor has ordered a test which requires you to be fasting prior to collection. Blood composition is significantly altered after meals and is not suitable for many clinical chemistry tests. Improper preparation for this test may cause the wrong answer to be given to the doctor, resulting in the wrong treatment to be given.

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COLLECTION OF SPECIMEN

  1. Abstain from all food for a minimum of 10 – 12 hours (14 hours for triglyceride analysis). Water may be consumed during this time.
  2. Check with your doctor to determine if you should continue to take your usual medication(s) during this time. The laboratory cannot advise you on medications.
  3. As soon as the laboratory has collected the required specimen, you may resume normal eating unless otherwise directed by your doctor.

If you have any questions about these instructions, please call the Client Service department at 219-989-3700 or 800-937-5521.

Glucose Tolerance Test

PURPOSE OF TEST
Your doctor has ordered a glucose tolerance test (GTT). The purpose of a GTT is to test the efficiency of your body’s insulin-releasing mechanism and glucose disposing system. You must prepare your body for the GTT by changing your eating and medication routines slightly for 3 days before the test. It is very important that you follow the instructions below in order for accurate results to be obtained. The test will last 3 – 5 hours depending on the doctor’s order. During that time you will be required to remain in the laboratory area.

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PREPARATION FOR THE TEST

MEDICATION: You must tell your doctor if you are currently taking any medications before proceeding with the GTT as some may interfere with test results

EXERCISE: Do not exercise for 12 hours before the GTT

DIET: For three days prior to the test, the recommended dietary intake of carbohydrate per day is 150 grams. Your doctor will advise you on a special dietary plan prior to the GTT.

COLLECTION OF SPECIMEN

  1. Call the laboratory to schedule the date of your test.
  2. You must be fasting the morning of the test. Abstain from eating or drinking anything except water for 8 – 10 hours prior to the start of the test. Check with your doctor regarding any medications and if you should take them prior to the test.
  3. When you arrive at the laboratory for the test, you will have a blood specimen collected. The lab may check the glucose level of this specimen before continuing on with the test.
  4. When the results of the baseline testing are complete, you will be given a liquid called “glucola” to drink. You must drink the entire amount given to you within 5 minutes.
  5. After you drink the glucola, you will have blood specimens collected at specified intervals. The most common intervals are at one, two, and three hours post glucose (glucola) dose.
  6. During the test, you must refrain from eating or drinking anything except water. You will not be able to leave the building during the test.

If you have any questions about these instructions, please call the Client Service department at 219-989-3700 or 800-937-5521.

Post Prandial Glucose

PURPOSE OF TEST
Your doctor has ordered a test for glucose to be collected two hours after you have eaten. It is important to eat a high carbohydrate diet 2-3 days prior to the test. The day of the test you should eat a meal containing approximately 100 grams of glucose.

NOTE: DIABETIC PATIENTS: The following diet is for patients not known to be a diabetic. Diabetic patients should follow their usual diet.

Read Instructions
COLLECTION OF SPECIMEN

  1. Listed below is an example of a breakfast and a lunch that meet the diet requirements of the test.
    BREAKFAST LUNCH
    1 cup of orange juice 1 cup of orange juice
    1 cup of cornflakes or oatmeal 1 bowl of vegetable soup
    1 cup of milk 4 saltine crackers
    1 tablespoon of sugar 1 slice of lunch meat or cheese
    2 slices of toast 2 slices of bread
    1 tablespoon of jelly 1 tablespoon margarine or mayonnaise
    1 egg (if desired) ½ cup vegetable salad
    Coffee or Tea (if desired) Coffee or Tea (if desired)
    1 scoop of ice cream
  2. Choose one of the meals; eat all of the food listed. Complete the meal within 15 minutes.
  3. Return to the lab 1 1/2 hours after you have completed the meal. Laboratory personnel will collect a blood specimen for a glucose level two (2) hours after you have completed your meal.

If you have any questions about these instructions, please call the Client Service department at 219-989-3700 or 800-937-5521.

Hemoccult Testing

PURPOSE OF TEST
Your doctor has ordered a test to determine if there is blood in your stool. The test is commonly called the Hemoccult Test or Fecal Occult Blood Test. This test requires you to follow a special diet for at least 48 hours prior to and during the collection period. The diet is designed to prevent a false positive test due to the food you have eaten.

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SPECIAL DIET INSTRUCTIONS

  1. Foods to EAT:
    • Well-cooked pork and fish, cooked fruits and vegetables, high fiber foods (whole wheat bread, bran cereal, popcorn).
  2. Foods, vitamins, and drugs to AVOID:
    • Red meat (beef, lamb) including processed meats and liver, any raw fruits and vegetables (especially melons, radishes, turnips and horseradish), Vitamin C in excess of 250 mg per day, aspirin or other non-steroidal anti-inflammatory drugs (avoid 7 days prior to and during the test period).
  3. Modifications which can be made ONLY if the patient cannot follow the above diet:
    • Moderate amounts of these raw fruits and vegetables:
      • Apples
      • Lettuce
      • Plums
      • Apricots
      • Oranges
      • Raisins
      • Bananas
      • Peaches
      • Raspberries
      • Celery
      • Pears
      • Strawberries
      • Tomatoes
    • Moderate amounts of alcoholic beverages.

LABELING THE SPECIMEN

Using a ballpoint pen, print the patient’s full name, age, and address on the front of each slide. After each collection, fill in the date on the front of the slide.

COLLECTION OF SPECIMENS

  1. Do not collect samples during, or until three days after your menstrual period, while you have bleeding hemorrhoids, or while blood is in your urine.
  2. Protect the slides from heat, light, and volatile chemicals (e.g. Iodine or bleach).
  3. Keep the cover of the slides closed when not in use.
  4. Follow the special diet instructions for at least 48 hours before collecting the first stool sample.
  5. Just before a bowel movement, flush the toilet and wait for the bowl to refill. Unfold the flushable collection tissue and float it on the surface of the water. Allow the edges to stick to the sides of the bowl. Let the stool fall onto the tissue. (NOTE: Do not be concerned if some water collects in the tissue.)
  6. Each card contains three (3) sections or slides. Each section contains two (2) windows for sample application.
    • Open the front of Slide 1
    • Use one stick to collect a small sample from the collection tissue.
    • Apply sample inside Box A. The specimen should be spread out inside each box. Do not leave the specimen in a thick clump on the slide.
    • Collect second sample from a different part of stool with same stick.
    • Apply sample inside Box B. The specimen should be spread out inside each box. Do not leave the specimen in a thick clump on the slide.
  7. Flush collection tissue; discard stick in a waste container. DO NOT FLUSH THE STICK.
  8. Close the cover flap of each box. Fill in the date on the front of the slide. Store slide in the paper envelope (not the return envelope), to allow completed slide to air-dry overnight.
  9. Repeat steps 6 – 9 for your next 2 bowel movements, using Slides 2 and 3. After the last completed slide has air-dried overnight, remove all slides from the paper envelope and place all slides in the mailing pouch provided. Seal pouch carefully and mail.

STORAGE OF SPECIMEN/DELIVERY TO LAB

Each kit envelope is pre-labeled with the laboratory address and postage. The envelope can be mailed or hand-delivered to the laboratory.

If you have any questions about these instructions, please call the Client Service department at 219-989-3700 or 800-937-5521.

Stool Specimen Collection

Package Insert for Personal Use Kit

Read all package insert directions carefully before sample collection.

Test results may be invalid if test is not performed properly

Read Instructions

Sample Deposit

  1. Place supplied collection paper inside toilet bowl on top of water.
  2. Deposit stool sample on top of collection paper.
  3. Collect sample from stool before paper sinks and stool sample touches water.
  4. Collection paper is biodegradable and will not harm septic systems.

Sample Collection

  1. Fill in all required information on the sampling bottle. Open green cap by twisting and lifting.
  2. Scrape the surface of the fecal sample with the sample probe. Cover the grooved portion of the sample probe completely
  3. Close sampling bottle by inserting the sample probe and snap green cap on tightly. Do not reopen. Return the sample device to your doctor or laboratory in envelope provided. Please do not mail to Polymedco.

      Pap Smear Collection Information

      Click here for information on what is being tested with a Pap smear

      View our brochure on the what’s and what-not to do before your Pap smear appointment

      GC/Chlamydia Female Self Collection Instruction

      GC/Chlamydia Female Self Collection Instruction – Vaginal Specimen Transport for the BD ProbeTec™ CT/GC Qx Amplified DNA Assays

      Please read all instructions before collecting specimens. If you have any questions about this procedure, please ask your doctor or nurse.

      Read Instructions

      Instructions for Self-Collection of Vaginal Swabs

      Please read all instructions before collecting specimens. If you have any questions about this procedure, please ask your doctor or nurse.

      1. Wash hands with soap and water. Rinse and dry.
      2. It is important to maintain a comfortable balance during the collection procedure.
      3. Twist the cap to break the seal (Figure 1). Do not use if seal is broken or damaged. Pull the cap with attached swab off the tube. Do not touch the soft tip or lay the swab down. If you touch or drop the swab tip or the swab is laid down, discard the swab and request a new vaginal swab.
      4. Hold the swab by the cap with one hand so the swab tip is pointing toward you (Figure 2).
      5. With your other hand, gently spread the skin outside the vagina. Insert the tip of the swab into the vaginal opening (Figure 2.) Point the tip toward your lower back and relax your muscles.
      6. Gently slide the swab no more than two inches into the vagina (Figure 3). If the swab does not slide easily, gently rotate the swab as you push. If it is still difficult, do not attempt to continue. Make sure the swab touches the walls of the vagina so that moisture is absorbed by the swab.
      7. Rotate the swab for 10-15 seconds (Figure 4).
      8. Withdraw the swab without touching the skin. Place the swab in the tube and cap securely (Figure 5).
      9. Repeat steps 2-8 if a second swab is to be collected.
      10. After collection, wash hands with soap and water, rinse, and dry.
      11. Return tube with swab as instructed.